FREE
AKTU Plant Biotechnology Lab (BBT652) Syllabus PDF – B.Tech 3rd Year
Plant Biotechnology Lab BBT652
All syllabus, notes, question bank & PYQ for this subject
Free
Download now
- English
- Semester 6
- 0 downloads
- 0 views
Official AKTU syllabus of Plant Biotechnology Lab (BBT652) for B.Tech 3rd Year – 1 credits, L-T-P 0-0-2. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Plant Biotechnology Lab
Download PDFBBT652 · 1 credits · L-T-P 0-0-2
EXAM DURATION: 2 Hours SEMESTER: VI (EVEN) L: T: P :: 0 : 0 : 2 CREDITS: 1 PREREQUISITE: Plant Biotechnology theory course
Course Objectives
- To provide knowledge to apply fundamental principles of plant tissue culture.
- To teach concepts behind culturing techniques from different explants.
- To inculcate the hands on practice attitude in students to perform explants selection, media preparation, sterilization and callus culture initiation. Course Outcomes (Six): On completion of this course, the students will be able to:
- Operate and handle the plant biotechnology lab equipment.
- Perform tissue culture media preparation, sterilization and explants selection.
- Understand in vitro cultures through axillary bud induction
- Analyze plant secondary metabolites from selected medicinal
- Learn DNA/RNA extraction and estimation
- Perform extraction of plant proteins along with estimation. CO-PO Mapping (1 to 3 scale)
| CO/PO | PO-1 | PO-2 | PO-3 | PO-4 | PO-5 PO-6 PO-7 PO-8 PO-9 PO- 10 PO- 11 PO- 12 |
| CO-1 | 3 | 1 | 2 | 3 | 1 2 1 2 |
| CO-2 | 2 | 1 | 3 | 1 | 2 |
| CO-3 | 2 | 1 | 3 | 1 | 2 1 |
| CO-4 | 2 | 1 | |||
| CO-5 | 2 | 1 | 3 | 2 | 1 |
| CO-6 | 2 | 3 | 2 | 1 |
Reference Books
| S. No. | Name Of Authors/Books/Publishers | Year of Publication/ Reprint |
| 1. | Plant Biotechnology: Practical Manual by C.C. Giri, ArchanaGiri I. K. International Publications. | 2007 |
| 2. | A Practical Manual For Plant Biotechnology by Tejovathi G, CBS Publishers and Distributors. | 1996 |
| 3. | Plant Biotechnology: Laboratory Manual For Plant Biotechnology by H.S. Chawla, Oxford and IBH Publishing | 2004 |
Course Details
| S. No | List of Experiments |
| 1. | Preparation of Stocks solution for plant tissue culture media. |
| 2. | Preparation of MS/B5 medium (semi-solid) and sterilization. |
| 3. | Explant selection, preparation and surface sterilization. |
| 4. | To learn culturing, sub culturing and maintenance using selected explants. |
| 5. | Initiation of in vitro cultures through axillary bud induction. |
| 6. | Initiation of callus cultures from different explants. |
| 7. | Preparation of artificial seed/synthetic seed for conservation of germplasm. |
| 8. | Extraction of DNA/RNA from plants and its estimation. |
| 9. | Isolation and characterization of plant secondary metabolites from selected medicinal plants. |
| 10. | Extraction of proteins from plants and its estimation. |
Ratings & Reviews
No reviews yet — share your experience with this item.