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AKTU Bioprocess Engineering II Lab (BBT651) Syllabus PDF – B.Tech 3rd Year
Bioprocess Engineering II Lab BBT651
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Official AKTU syllabus of Bioprocess Engineering II Lab (BBT651) for B.Tech 3rd Year – 1 credits, L-T-P 0-0-2. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Bioprocess Engineering II Lab
Download PDFBBT651 · 1 credits · L-T-P 0-0-2
II Lab EXAM DURATION: 2 Hours SEMESTER: VI (EVEN) L: T: P :: 0 : 0 : 2 CREDITS: 1 PREREQUISITE: Bioprocess Engineering theory course
Course Objectives
- To impart knowledge about the basic fundamental principles of bioprocess engineering by performing different experiments.
- To make them correlate theory and practical process by experimentation. Course Outcomes (Six): On successful completion of the course, the student will be able to
- Learn process of cellular disruption and cell components separation
- Learn estimation and separation of proteins using different techniques.
- Understand the process of manufacturing and processing of recombinant proteins.
- Understand the basics of different chromatographic techniques and their applications in manufacturing industries.
- Learn the process of isolation and extraction of phytochemicals
- Learn product polishing techniques. CO-PO Mapping (1 to 3 scale)
| CO/PO | PO-1 | PO-2 PO-3 PO-4 PO-5 PO-6 PO-7 PO-8 PO-9 PO- 10 PO- 11 PO- 12 |
| CO-1 | 2 | 1 3 1 |
| CO-2 | 2 | 3 1 2 1 |
| CO-3 | 1 | 2 |
| CO-4 | 3 | 2 1 |
| CO-5 | 2 | 1 |
| CO-6 | 2 | 1 |
Reference Books
| S. No. | Name Of Authors/Books/Publishers | Year of Publication/ Reprint |
| 1. | Scopes, R. K. Protein Purification: Principles and Practice, 3rd Edn, Springer. | 2013 |
| 2. | Andreas, H., Walker, J. M., Wilson, K., Clokie, S. (Eds.). Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, United Kingdom: Cambridge University Press. | 2018 |
| 3. | Belter, P. A., Cussler, E. L., and Hu, W.S. Bioseparation: Downstream Processing for Biotechnology, 2nd Edn., Wiley-Interscience. | 1994 |
| 4. | Abelson, J. N. Simon, M. I. and Deutscher, M. P. Methods in Enzymology: Guide to Protein Purification, Volume 182, Academic Press. | 1990 |
| 5. | Published research articles related to the experiments |
Course Details
| S. No | List of Experiments |
| 1 | Estimation and separation of proteins using different techniques. |
| 2 | Bacterial cell disruption using different methods like physical, chemical and enzymatic methods. |
| 3 | Downstream processing of a recombinant protein over expressed in bacterial system. |
| 4 | Separation of a recombinant protein in bacterial cell lysate using Ammonium sulphate precipitation. |
| 5 | High resolution purification of the recombinant protein by affinity chromatography. |
| 6 | Downstream processing of lysozyme from egg white using ion exchange chromatography. |
| 7 | Downstream processing of lysozyme from egg white using ion exchange chromatography. |
| 8 | Downstream processing of caffeine from tea dust. |
| 9 | Experiments involving crystallization of the downstream processed material. |
| 10 | Product polishing by lyophilisation and drying. |
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