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AKTU Genetic Engineering Lab (BBT551) Syllabus PDF – B.Tech 3rd Year
Genetic Engineering Lab BBT551
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Official AKTU syllabus of Genetic Engineering Lab (BBT551) for B.Tech 3rd Year – 1 credits, L-T-P 0-0-2. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Genetic Engineering Lab
Download PDFBBT551 · 1 credits · L-T-P 0-0-2
Course Objectives
To isolate the various biomolecules and genetic materials from cells and tissues To develop understanding of estimation of Genetic material To provide practical knowledge restriction digestion, transformation, screening and verification of cloning Practical knowledge of ligation, blotting and cloning.
COURSE OUTCOMES (SIX): Upon completion of this course, the students will be able to:
| CO1 | Demonstrate the isolation genetic materials |
| CO2 | Perform experiments related to cloning, ligation, restriction digestion and transformation etc. |
| CO3 | Demonstrate the Southern Blotting for identification of desired DNA in a pool DNA samples |
| CO4 | Perform the bacterial cell competent for transformation |
| CO5 | To be able to express foreign protein |
| CO6 | To be able to use acquired knowledge for commercial products |
CO-PO MAPPING (1 TO 3 SCALE)
| CO/PO | PO-1 | PO-2 | PO-3 | PO-4 PO-5 PO-6 PO-7 PO-8 PO-9 PO- 10 PO- 11 PO- 12 |
| CO-1 | - | 3 | - | 3 |
| CO-2 | - | 3 | - | 3 |
| CO-3 | - | 3 | - | 3 |
| CO-4 | - | 3 | - | 3 |
| CO-5 | - | 3 | - | 3 |
| CO-6 | - | 3 | - | 3 |
Reference Books
- Laboratory manual on Molecular Biology & genetic Engineering-A new approach (2012), R.S. Sengar
- Laboratory Manual for Genetic Engineering (2009) S. john Vennison. Prentics hall publication
| COURSE DETAILS: Genetic Engineering Lab | |
| S. NO. | LIST OF EXPERIMENT |
| 1 | Isolation of RNA and its estimation by orcinol method |
| 2 | Isolation of plasmid DNA and its estimation by diphenylamine reaction |
| 3 | Elution of plasmid DNA from agarose gel |
| 4 | To perform restriction digestion of λ DNA |
| 5 | Dephosphorylation of restriction enzyme digested vector pUC18 |
| 6 | To make bacterial cells competent for transformation |
| 7 | To perform of transformation of the desired bacterial strain with plasmid DNA |
| 8 | Screening of transformed colonies by X gal and IPTG |
| 9 | Verification of cloning by colony PCR and screening of the positive colonies |
| 10 | To perform a Southern Blotting for identification of desired DNA in a pool DNA samples |
| 11 | To perform ligation of λ EcoRI digest using T4DNA ligase |
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