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AKTU Genetic Engineering (BBT501) Syllabus PDF – B.Tech 3rd Year
Genetic Engineering BBT501
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Official AKTU syllabus of Genetic Engineering (BBT501) for B.Tech 3rd Year – 4 credits, L-T-P 3-1-0. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Genetic Engineering
Download PDFBBT501 · 4 credits · L-T-P 3-1-0
Course Objectives
To Provide knowledge of manipulation of Genetic Material and Recombinant Technology To teach the construction of genomic c-DNA libraries, cloning and strain improvement To develop understanding of DNA sequencing, Molecular markers and related techniques.
Application of Genetic Engineering and its application To impart knowledge of cell signaling and Ethical issues COURSE OUTCOMES (SIX): Upon completion of this course, the students will be able to:
| CO1 | To be able to appraise the appropriate use of host and vector for gene cloning |
| CO2 | Identification of appropriate method for DNA delivery into the host |
| CO3 | Use of gene library for screening of desired gene sequence/protein |
| CO4 | Cloning process of whole organism and its application |
| CO5 | Process of recombinant protein expression, cell signaling and ethical issues related to Gene transfer |
| CO6 | To be able to use acquired knowledge for commercial products |
CO-PO MAPPING (1 TO 3 SCALE)
| CO/PO | PO-1 | PO-2 | PO-3 | PO-4 | PO-5 | PO-6 | PO-7 | PO-8 PO-9 PO- 10 PO- 11 PO- 12 |
| CO-1 | 1 | 2 | 1 | 2 | 1 | 3 | 1 | 2 3 |
| CO-2 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 3 |
| CO-3 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 1 2 3 |
| CO-4 | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 3 |
| CO-5 | 3 | 2 | 3 | 2 | 1 | 2 | 1 | 3 |
| CO-6 | 1 | 3 | 1 | 2 | 3 | 2 | 1 | 3 |
Reference Books
- T.A Brown (2006). Gene cloning and DNA analysis, WILEY- BLACKWELL
- Molecular Biology of the Cell (2006) Bruce Alberts.6th edition
- Molecular Cloning, (2001) A laboratory Manual. Sambrook, J., Fritsch, E.F., Mariatis.3rd edition (Vol.1,2,3)
- S.B Primrose (2001). Molecular biotechnology. Panama Publishing corporation, 2nd edition
- Genetic Engineering (2009) Dr Smita Rastogi & Dr Neelam Pathak, Oxford University Press
| COURSE DETAILS: GENETIC ENGINEERING | ||
| UNITS | CONTENTS | LECTURE HOURS |
| I | Manipulation of DNA – Restriction and Modification enzymes, Design of linkers and adaptors. Characteristics of cloning and expression vectors based on plasmid and bacteriophage, Vectors for yeast, insect and mammalian systems, Prokaryotic and eukaryotic expression host systems, Tissue specific promoter, wound inducible promoters, Strong and regulatable promoters, promoter analysis (EMSA and DNA foot printing); Introduction of recombinant DNA in to host cells and selection methods. | 8 |
| II | Construction of genomic and cDNA libraries, Artificial chromosomes – BACs and YACs, Chromosome walking, Screening of DNA libraries using nucleic acid probes and antisera., strain improvement of industrially important organisms, CRISPER / Cas system of gene editing. | 8 |
| III | Maxam Gilbert’s and Sanger Coulson’s and automated methods of DNA sequencing, Inverse PCR, Nested PCR, AFLP-PCR, Allele specific PCR, Assembly PCR, Asymmetric PCR, Hot start PCR, Colony PCR, single cell PCR, Real-time PCR/qPCR – SYBR green assay, TaqMan assay, Molecular beacons, Applications of PCR; Site directed mutagenesis.; molecular markers (RAPD, RFLP, AFLP, SNP) | 8 |
| IV | Applications of genetic engineering; cloning of sheep (Dolly) & other mammals; applications in conservation; therapeutic vs. reproductive cloning; ethical issues and the prospects for human cloning; Gene therapy; DNA drugs and vaccines. | 8 |
| V | Basic concepts of cell signaling, Extracellular signal molecule and their receptors, Operation of Signaling molecules over various distances, Cellular response to specific combinations of extracellular signal molecules; Nuclear receptor; Ion channel linked, G-protein mediated receptors, Relay of signal by activated cell surface receptors via intracellular signaling proteins, Intracellular Signaling proteins as molecular switches. 40 | 8 |
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