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AKTU Plant Biotechnology (BBT602) Syllabus PDF – B.Tech 3rd Year
Plant Biotechnology BBT602
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Official AKTU syllabus of Plant Biotechnology (BBT602) for B.Tech 3rd Year – 4 credits, L-T-P 3-1-0. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Plant Biotechnology
Download PDFBBT602 · 4 credits · L-T-P 3-1-0
EXAM DURATION:3 HOURS SEMESTER: VI (EVEN) L: T: P :: 3 : 1 : 0 CREDITS: 4 PREREQUISITE: Basic knowledge of genetic engineering ,biochemistry and elementary biology
Course Objectives
- To impart the basic concepts of plant tissue culture.
- To develop understanding about tissue culture techniques and involved culturing strategies.
- To impart knowledge about the importance of tissue culture in crop improvement.
Course Outcomes
On successful completion of the course, the student will be able to:
- Understand the principle and basic requirements for plant tissue culture.
- Explain the difference between tissue and organ culture and their applicability.
- Understand haploid culture and in vitro selection of mutants.
- Analyse somaclonal variation for improved crop varieties in vitro cultures.
- Identify suitable cryopreservation and reculture technique for the cultured tissue.
- Understand the development of transgenic plants through genetic manipulations. CO-PO Mapping (1 to 3 scale)
| CO/PO | PO-1 | PO-2 | PO-3 | PO-4 | PO-5 | PO-6 PO-7 PO-8 PO-9 PO- 10 PO- 11 PO- 12 |
| CO-1 | 2 | 1 | 2 | 1 | 2 | 1 |
| CO-2 | 3 | 2 | 1 | 3 | 1 | |
| CO-3 | 2 | 1 | 2 | 1 | 3 | 1 |
| CO-4 | 2 | 1 | 3 | 1 | ||
| CO-5 | 2 | 1 | 2 | 1 | 3 | 1 |
| CO-6 | 2 | 1 | 3 | 1 |
Reference Books
| S. No. | Name Of Authors/Books/Publishers | Year of Publication/ Reprint |
| 1. | Hudson T Hartmann: Plant Propagation-Principle and Practices, Pearson Education India; 8 edition. | 2015 |
| 2. | Principles of Plant Biotechnology- An Introduction of Genetic Engineering in Plants by S.H. Mantell, J.W. Mathews and R.A. Mckee, Blackwell Scientific Publications. | 1985 |
| 3. | Chopra V L, Sharma R P & Swaminathan M S: Agricultural Biotechnology by Science Pub Inc. | 1996 |
| 4. | Hamish A, Collin & Sue Edwards: Plant Cell Culture, BIOS Scientific Publishers. | 1998 |
| 5. | Razdan M K: An Introduction to Plant Tissue Culture, Science Publishers. | 2003 |
| 6. | Plant Tissue Culture: Theory and Practice by S.S. Bhojwani M.K. Razdan, Elsevier Science. | 1996 |
| 7. | H.S. Chawla. Plant Biotechnology, Oxford & IBH Publishing 2020Bioprocess Technology - Kinetics & Reactors" by A Moser, Springer-Verlag. | 1998 |
Course Details
| Course Details: PLANT BIOTECHNOLOGY | ||
| Unit | Content | Contact Hours |
| I | Introductory history of plant biotechnology: Laboratory organization; Principles of Plant Tissue Culture. Concepts of totipotency, explants, inoculums, acclimatization. Nutrition of plant cells; Nutrient media: Composition of commonly used nutrient culture media with respect to their contents like inorganic chemicals, organic constituents. An appraisal of different media, selection of media, Sterilization of the media. Hormones: Auxins, Cytokinins, Gibberellins, Abscisic Acid, Ethylene etc. Explant preparation and Surface sterilization. Basic procedure for Aseptic Tissue transfer. | 10 |
| II | Culture of plant materials- explants selection and technique of culturing. Organogenesis, Embryogenesis, Somaclonal variation, germiclonal variation. Establishment, growth and maintenance of Callus and cell suspension culture, Methods of sub culturing and transfer of regenerated plants to the field. Tissue and organ culture; Cellular differentiation and regulation of morphogenesis; Somatic embryogenesis; Control of organogenesis and embryogenesis; Single cell culture | 10 |
| III | Haploid production: Androgenesis; Anther and microspore culture; Gynogenesis; Embryo culture and rescue in agricultural and horticultural corps; Protoplast isolation; Culture– regeneration; Somatic hybrid-cybrids; In vitro selection of mutants – mutants for salts, disease, cold, drought, herbicide and other stress conditions; Micropropagation: Application of micropropagation in agriculture and forestry. Meristem culture and virus elimination; Shoot tip culture. | 8 |
| IV | Improved crop varieties through somaclonal variation in invitro cultures. Application of tissue culture for crop improvement in agriculture, horticulture and forestry. Cryopreservation and slow growth cultures, Freezing and storage, thawing, reculture. Application of plant tissue culture production of secondary metabolites and other industrial products. | 8 |
| V | Genetic transformation using Ti plasmid Manipulation of gene expression in plants; Production of marker free transgenic plants. Developing insect-resistance, diseaseresistance, herbicide resistance plants. Genetic manipulation of flower pigmentation, Developing quality of seed storage, Provitamin A, iron proteins in rice, modification of food plant taste and appearance, yield increase in plants. 44 | 8 |
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