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AKTU Animal Biotechnology (BBT061) Syllabus PDF – B.Tech 3rd Year
Animal Biotechnology BBT061
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Official AKTU syllabus of Animal Biotechnology (BBT061) for B.Tech 3rd Year – 3 credits, L-T-P 3-0-0. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Animal Biotechnology
Download PDFBBT061 · 3 credits · L-T-P 3-0-0
EXAM DURATION: 3 Hours SEMESTER: VI (EVEN) L: T: P :: 3 : 0 : 0 CREDITS: 3 PREREQUISITE: Basic Knowledge of Genetic Engineering and Immunology
Course Objectives
- To introduce in vitro culture techniques of animal cells and tissues
- To learn different types of culture systems and reactors used for culturing of animal cells
- To elaborate various applications of animal tissue cultures with specific reference to transgenic animal production Course Outcomes (Six): On completion of this course, the students will be able to:
- Understand basics of animal tissue culture and its importance
- Learn about the different types of animal cell culture media, their maintenance and cultivation of cell lines.
- Understand techniques to establish animal cell cultures invitro as well as different types of reactors and their working
- Learn the strategies involved in developing clones in lab
- Understand the methods of transgene delivery and production of transgenic animals
- Understand the process of stem cell differentiation and their applications with case studies CO-PO Mapping (1 to 3 scale)
| CO/PO | PO-1 | PO-2 | PO-3 PO-4 PO-5 PO-6 PO-7 PO-8 PO-9 PO- 10 PO- 11 PO- 12 |
| CO-1 | 1 | 2 | 1 |
| CO-2 | 2 | 1 | 2 |
| CO-3 | 2 | 1 | 2 1 |
| CO-4 | 3 | 2 | 1 2 |
| CO-5 | 3 | 2 | 1 3 1 2 1 |
| CO-6 | 3 | 2 | 1 3 1 2 1 |
Reference Books
| S. No. | Name Of Authors/Books/Publishers | Year of Publication/ Reprint |
| 1. | B. Hafez and E.S.E Hafez, Reproduction in farm animals, 7th Edition, Wiley Blackwell | 2000 |
| 2. | G.E. Seidel, Jr. and S.M. Seidel, Training manual for embryo transfer in cattle (FAO Animal Production and Health Paper-77), 1st Edition, W.D. Hoard and sons FAO | 1991 |
| 3. | I. Gordon, Laboratory production of cattle embryos, 2nd edition, CAB International | 2003 |
| 4. | I. Gordon, Laboratory production of cattle embryos, 2nd edition, CAB International | 1997 |
| 5. | Animal cell culture: Ian Freshney | 2015 |
Course Details
| COURSE DETAILS: ANIMAL BIOTECHNOLOGY | ||
| Unit | Content | Contact Hours |
| I | Basic cell culture techniques, Types of cell culture media; Ingredients of media; Physiochemical properties; CO2 and bicarbonates; Buffering; Oxygen; Osmolarity; Temperature; Surface tension and foaming; Balance salt solutions; Antibiotics growth supplements; Foetal bovine serum; Serum free media; Trypsin solution; Selection of medium and serum; Conditioned media; Other cell culture reagents; Preparation and sterilization of cell culture media, serum and other reagents. | 8 |
| II | Different tissue culture techniques; Types of primary culture; Chicken embryo fibroblast culture; Chicken liver and kidney culture; Secondary culture; Trypsinization; Cell separation; Continuous cell lines; Suspension culture; Organ culture etc.; Behaviour of cells in culture conditions: division, growth pattern, metabolism of estimation of cell number; Development of cell lines; Characterization and maintenance of cell lines, stem cells; Cryopreservation; Common cell culture contaminants | 8 |
| III | Cell cloning and selection; Transfection and transformation of cells; Commercial scale production of animal cells, stem cells and their application; Application of animal cell culture for in vitro testing of drugs; Testing of toxicity of environmental pollutants in cell culture; Application of cell culture technology in production of human and animal viral vaccines and pharmaceutical proteins | 8 |
| IV | Cell culture reactors; Scale-up in suspension; Scale and complexity; Mixing and aeration; Rotating chambers; Perfused suspension cultures; Fluidized bed reactors for suspension culture; Scale-up in monolayers; Multi-surface propagators; Multi-array disks, spirals and tubes; Roller culture; Micro carriers; Perfused monolayer cultures; Membrane perfusion; Hollow fibre perfusion; Matrix perfusion; Microencapsulation; Growth monitoring | 8 |
| V | Transgenic animal production; Methods of transgene delivery; Integration of foreign genes and their validation; Gene targeting; Methods and strategies; Improving transgene integration efficiency; Cell lineages and developmental control genes in drosophila and mice; Differentiation of germ layers; Cellular polarity; Stem cell differentiation; Blood cell formation; Fibroblasts and their differentiation; Differentiation of cancerous cells and role of proto oncogenes 40 | 8 |
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