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AKTU Recombinant DNA Technology Lab (BBI551) Syllabus PDF – B.Tech 3rd Year
Recombinant DNA Technology Lab BBI551
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- Semester 5
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Official AKTU syllabus of Recombinant DNA Technology Lab (BBI551) for B.Tech 3rd Year – 1 credits, L-T-P 0-0-2. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Recombinant DNA Technology Lab
Download PDFBBI551 · 1 credits · L-T-P 0-0-2
Course Outcomes
At the end of the course, the student will be able to:
| CO1 | Apply restriction digestion, ligation, and PCR techniques for DNA manipulation. | K3 |
| CO2 | Prepare competent cells, perform transformation in E. coli, and examine recombinant clones. | K3 |
| CO3 | Isolate genomic and plasmid DNA, purify digested fragments, and confirm insert integration. | K3 |
| CO4 | Examine recombinant constructs using agarose gel electrophoresis, PCR products, and blue-white screening. | K3 |
| CO5 | Demonstrate practical skills in recombinant DNA technology workflows from DNA isolation to gene amplification. | K3 |
List of Experiments
- Restriction enzyme digestion of plasmid
- Purification of digested DNA
- Ligation of DNA fragment with plasmid DNA
- Preparation of competent cells
- Transformation in E. coli
- Isolation of recombinant plasmid and confirmation of insert DNA
- Blue-white screening of transformants
- Polymerase Chain Reaction
- Sanger Sequencing
- Next Generation Sequencing Note: The instructor may add / delete / modify / tune experiments as required.
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