FREE
AKTU Recombinant DNA Technology (BBI501) Syllabus PDF – B.Tech 3rd Year
Recombinant DNA Technology BBI501
All syllabus, notes, question bank & PYQ for this subject
Free
Download now
- English
- Semester 5
- 0 downloads
- 0 views
Official AKTU syllabus of Recombinant DNA Technology (BBI501) for B.Tech 3rd Year – 4 credits, L-T-P 3-1-0. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Recombinant DNA Technology
Download PDFBBI501 · 4 credits · L-T-P 3-1-0
Course Outcomes
At the end of the course, the student will be able to:
| CO 1 | Understand vectors and insert preparation (plasmids, phages, cosmids, BAC, YAC; restriction digestion, PCR). | K2 |
| CO 2 | Apply cloning techniques (ligation, modification enzymes, transformation, transfection, and screening). | K3 |
| CO 3 | Construct gene libraries (cDNA, subtractive cDNA, genomic, BAC, YAC). | K3 |
| CO 4 | Apply methods for expression of recombinant proteins in E. coli (expression vectors, promoters, reading frame, purification tags). | K3 |
| CO 5 | Understand the applications of DNA technology in diagnostics, therapeutics, agriculture, and genome editing, including AI-based rapid diagnostic technologies. | K2 |
Course Content
L-T-P: 3-1-0
| Unit | Topic | Proposed Lecture |
|---|---|---|
| I | VECTORS AND INSERT: Vehicles for gene cloning - Biology of plasmids, Plasmid based vectors, Bacteriophage, Phage vectors, Cosmids, Phasmids, BAC and YAC vectors; Insert preparation- Restriction Digestion-Restriction enzymes, Polymerase chain reaction –Polymerase, Creation of restriction site by PCR. | 08 |
| II | CLONING TECHNIQUE: Ligation –Ligase, cohesive, Blunt end ligation; Other Modification enzymes – Nucleases, Phasphatase, Kinase; Transformation-Preparation of competent cell, chemical and electrical transformation; Transfection; Recombinant selection and screening -Blotting technique. | 08 |
| III | CONSTRUCTION OF GENE LIBRARIES: Construction of cDNA library- construction subtractive cDNA library – construction of genomic DNA library – BAC library – YAC library. | 08 |
| IV | EXPRESSION OF RECOMBINANT PROTEIN IN E. COLI: Plasmid expression vectors-general features, promoters used in expression vectors -cloning of genes in correct reading frame in expression vector- purification of recombinant protein using Histidine tag, GST tag, chitin. | 08 |
| V | APPLICATION OF DNA TECHNOLOGY: Applications of rDNA technology in Diagnostics and Therapeutics - HIV diagnosis, Human Insulin, Growth hormone; Recombinant Vaccines, Agricultural Technologies - Golden rice, Insect resistant crops, Genome Editing Tools using CRISPR Cas9 – Principle mechanism application ethics; Artificial Intelligence Application in RDT. | 08 |
Text Books
- Jerney D. Wale and Malcolm Von Schants, “From Genes to Genomes: Concepts and Applications of DNA Technology”, John Wiley & Sons, 2002.
- Sandy B. Primrose, Richard M. Twyman, “Principles of gene manipulation”, 7th edition, Blackwell Publishing, 2006.
- T A Brown, Gene cloning and DNA analysis, 6th edition, Blackwell Publishing, 2010.
Ratings & Reviews
No reviews yet — share your experience with this item.