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AKTU Techniques in Biotechnology Lab (BBT351) Syllabus PDF – B.Tech 2nd Year
Techniques in Biotechnology Lab BBT351
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- Semester 3
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Official AKTU syllabus of Techniques in Biotechnology Lab (BBT351) for B.Tech 2nd Year – 1 credits, L-T-P 0-0-2. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Techniques in Biotechnology Lab
Download PDFBBT351 · 1 credits · L-T-P 0-0-2
Course Outcomes
| CO | Course Outcome |
|---|---|
| CO 1 | Understand concept of precision, accuracy for principle and working of laboratory microscope. |
| CO 2 | Learn and apply the spectrophotometric techniques for identification or quantification of biomolecules. |
| CO 3 | Understand the principle and execute the different chromatographic techniques for separation of biomolecules. |
| CO 4 | Apply the electrophoresis for quantitative and qualitative analysis of biomolecules |
| CO 5 | Demonstrate the extraction and separation of biomolecules. |
List of Experiments
- Demonstration of basic concept of precision and accuracy using appropriate experimental data
- Study of Beer-Lambert’s law-using UV-Visible spectrophotometer.
- To study principle and working of laboratory microscope.
- To analyze the isolated plant pigments using paper chromatography.
- Separation of amino acids using thin layer chromatography.
- Separation of a mixture of polar and non polar compounds using column chromatographic technique.
- To study and analysis of DNA sample by agarose gel electrophoresis.
- To study and analysis of protein sample by SDS- PAGE
- To study the separation of compounds using liquid-liquid extraction experiments.
- To study the separation of biological compounds using various membrane separation.
Text Books
- Wilson and Walker, “Principles and Techniques of Practical Biochemistry” 4 Edn., Cambridge Knew pros 1997.
- Biotechniques: Theory & Practice: Second Edition by SVS Rana, Rustogi Publications.
- Biochemical Methods of Analysis: Saroj Dua And Neera Garg: Narosa Publishing House, New Delhi.
- Bioanalytical Techniques : ML Srivastava; Narosa Publishing House, New Delhi.
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