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AKTU Enzyme Engineering (BBT403) Syllabus PDF – B.Tech 2nd Year
Enzyme Engineering BBT403
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Official AKTU syllabus of Enzyme Engineering (BBT403) for B.Tech 2nd Year – 3 credits, L-T-P 2-1-0. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Enzyme Engineering
Download PDFBBT403 · 3 credits · L-T-P 2-1-0
Course Outcomes
| CO | Course Outcome |
|---|---|
| CO 1 | Understand the concepts of enzyme and their kinetics. |
| CO 2 | Explain the factors affecting enzymatic reactions and role of inhibitors. |
| CO 3 | Perform the extraction, purification & characterization of different enzyme from different source. |
| CO 4 | Understand the concept of enzyme immobilization and its applications. |
| CO 5 | Apply knowledge of enzyme in developing biosensors and bioreactors. |
Detailed Syllabus
| Unit | Topic | Proposed Lectures |
|---|---|---|
| I | Introduction to enzymes: Holoenzyme, apoenzyme, prosthetic group. Interaction between enzyme and substratelock and key model, induced fit model. Features of active site, activation energy, enzyme specificity and types. IUB system of classification and nomenclature of enzymes. Kinetics of single substrate reactions; Derivation of Michaelis - Menten equation, turnover number; determination of Km and Vmax (LB plot, ED plot), Importance of Km &Vmax; Numerical related to enzyme kinetics, Multi-Substrate reaction mechanisms. | 08 |
| II | Factors affecting the velocity of enzyme catalyzed reaction- enzyme concentration, temperature, pH, substrate concentration, inhibitors and activators. Enzyme inhibition: irreversible; reversible (competitive, uncompetitive and non competitive inhibition); Substrate and Product inhibition, Allosteric regulation of enzymes, concerted & sequential model; Deactivation Kinetics. | 08 |
| III | Extraction of crude enzyme from plant, animal and microbial source; some case study. Purification of enzymes by the help of different methods. Methods of characterization of enzymes; criteria of purity. Unit of enzyme activity - definition and importance. Development of enzyme assays. | 08 |
| IV | Enzyme Immobilization: Adsorption, Matrix entrapment, Encapsulation, Cross linking, Covalent binding and their examples; Advantages and disadvantages of different immobilization techniques. Structure & stability of immobilized enzymes, kinetic properties of immobilized enzymes- partition effect, diffusion effect. Overview of applications of immobilized enzyme systems. | 08 |
| V | Enzyme Biosensors: elements of biosensors, three generations of biosensors, Types of biosensors: calorimetric, potentiometric, amperometric, optical and piezoelectric. Design of enzyme electrodes and their applications as biosensors in industry, health care and environment. Design of Immobilized Enzyme Reactors- Stirred tank reactors(STR), Continuous Flow Stirred Tank Reactors (CSTR), Packed- bed reactors (PBR), Fluidized-bed Reactors (FBR); Membrane reactors. | 08 |
Text Books
- Fundamentals of enzymology by Nicolas C. price and Lewis stevens. Oxford University Press
- Enzymes by Trevor palmer, East west Press
- Enzyme Technology by Messing.
- Enzymes: Dixon and Webb. (IRL Press)
- Enzyme technology by Chaplin and Bucke. Cambridge Univerity Press
- Biochemical engineering fundamentals, second edition. James E Bailey, David F., Ollis, McGraw Hill Intl. Edition
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