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AKTU Genetics and Molecular Biology Lab (BBT452) Syllabus PDF – B.Tech 2nd Year
Genetics and Molecular Biology Lab BBT452
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- Semester 4
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Official AKTU syllabus of Genetics and Molecular Biology Lab (BBT452) for B.Tech 2nd Year – 1 credits, L-T-P 0-0-2. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Genetics and Molecular Biology Lab
Download PDFBBT452 · 1 credits · L-T-P 0-0-2
Course Outcomes
| CO | Course Outcome |
|---|---|
| CO 1 | Calculate the allelic frequencies of numeric problems. |
| CO 2 | Isolate DNA from plant cell, plasmid DNA and bacterial genomic DNA from. |
| CO 3 | Determine the Tm of DNA and analyze the concentration of DNA in a given cell using spectrophotometer. |
| CO 4 | Purify the DNA using electrophoresis and its visualization under transilluminator. |
| CO 5 | Separate DNA using PAGE and amplification of DNA using polymerase chain reaction. |
List of Experiments
- How to calculate genetics and allelic frequencies numeric problem analysis.
- Isolation of Plasmid DNA.
- Isolation of Plant DNA.
- Estimation of DNA content in the given sample by spectrophotometer.
- Determination of Tm of DNA.
- Isolation of bacterial genomic DNA.
- Purification of DNA through Electrophoresis & visualization under UV transilluminator.
- Polyacrylamide gel electrophoresis of DNA.
- PCR amplification of DNA and visualization by gel electrophoresis.
- Isolation and study of polytene chromosome in Drosophila.
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