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AKTU Microbiology and Immunology Lab (BBT352) Syllabus PDF – B.Tech 2nd Year
Microbiology and Immunology Lab BBT352
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Official AKTU syllabus of Microbiology and Immunology Lab (BBT352) for B.Tech 2nd Year – 1 credits, L-T-P 0-0-2. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Microbiology and Immunology Lab
Download PDFBBT352 · 1 credits · L-T-P 0-0-2
Course Outcomes
| CO | Course Outcome |
|---|---|
| CO 1 | Apply different sterilization method and inoculation methods. |
| CO 2 | Demonstrate different staining procedures and culturing of microbes. |
| CO 3 | Exhibit different methodology for colony counting. |
| CO 4 | Understand the principle of blood grouping and immunodiffusion. |
| CO 5 | Apply ELISA and electrophoresis. |
List of Experiments
- LIST OF EXPERIMENTS
- Preparation of nutrient agar slants, plates and nutrient broth and their sterilization. (Microwave Oven,
- Heating mantles, Fridge, Heating Oven, Tube racks)
- Inoculation of agar slants, agar plate and nutrient broth (Incubators, Water bath, Laminar hood, dry heat
- sterilizer i.e. bead sterilizer)
- Culture of microorganisms using various techniques. (Shakers i.e. Cooling and Open shaker).
- Simple and differential staining procedures, endospore staining, flageller staining, cell wallstaining, capsular
- staining, negative staining. (Moist chambers, spirit lamps, slides, loops & microscopes, haemocytometer)
- Bacterial colony counting. (Moist chambers, spirit lamps, slides, loops & microscopes, haemocytometer)
- Isolation of microbes from soil samples and determination of the number of colony forming units. (U.V.
- spectrophotometer, Colony counter etc.)
- To determine the blood group and Rh of given blood sample.
- To perform single radial immunodiffusionanddoubleimmunodiffusion
- To perform rocket immune electrophoresis
- To perform counter current immune electrophoresis
- To perform ELISA
Text Books
- Lab Manual in microbiology by P Gunasekaran (New Age Int. Pub.).
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