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AKTU Biochemistry Lab (BBT353) Syllabus PDF – B.Tech 2nd Year
Biochemistry Lab BBT353
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Official AKTU syllabus of Biochemistry Lab (BBT353) for B.Tech 2nd Year – 1 credits, L-T-P 0-0-2. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Biochemistry Lab
Download PDFBBT353 · 1 credits · L-T-P 0-0-2
Course Outcomes
| CO | Course Outcome |
|---|---|
| CO 1 | Understand the basics of molarity, normality, buffers and pH meter. |
| CO 2 | Demonstrate the basics of titration and carbohydrate estimation. |
| CO 3 | Exhibit the difference between reducing and non-reducing sugars and estimation |
| CO 4 | Perform the extraction method of lipids and proteins on the basis of isoelectric point. |
| CO 5 | Understand the significance of chromatography. |
List of Experiments
- Preparation of solutions:
- (i) | Percentage solutions,
- (ii) | Molar solutions,
- (iii) | Normal solutions
- Titration of weak acid-weak base
- To perform quantitative estimation of carbohydrates
- To distinguish reducing and non-reducing sugars
- To perform quantitative estimation of proteins
- To estimate nucleic acids
- To perform isoelectric precipitation
- To separate sugars, fatty acids and amino acids by paper chromatography
- To extract lipids from plant material
- To perform thin layer chromatography
Text Books
- Wilson and Walker, “Principles and Techniques of Practical Biochemistry”, 4 Edn., Cambridge Knew pros 1997.
- Plummer DT, “An Introduction to Practical Biochemistry”, Ill Edn., Tata McGraw hill.
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