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AKTU Bioprocess Engineering Lab (BBT451) Syllabus PDF – B.Tech 2nd Year

Bioprocess Engineering Lab BBT451

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  • Semester 4
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Official AKTU syllabus of Bioprocess Engineering Lab (BBT451) for B.Tech 2nd Year – 1 credits, L-T-P 0-0-2. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.

Syllabus — Bioprocess Engineering Lab

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BBT451 · 1 credits · L-T-P 0-0-2

Course Outcomes

COCourse Outcome
CO 1Demonstrate the growth pattern and death kinetics of E. coli.
CO 2Discuss the upstream and downstream bioprocessing for product formation
CO 3Analyze the mass transfer concepts in bioprocess.
CO 4Perform immobilization of enzymes and microbial cell.
CO 5Develop computational design for fermentative production.

List of Experiments

  1. Determine the growth patterns and specific growth rate of E.coli.
  2. Determine the effect of peptone concentration on E.coligrowth .
  3. Determination of specific thermal death rate constant (Kd) for E.Coli.
  4. Determine the effects of temperature & pH on Psuedomonasputida
  5. Upstream and Downstream of bioprocess for the production of Citric acid by Aspergillusniger.
  6. Citric acid production from whey with glucose as supplementary carbon source by
  7. Aspergillusniger.
  8. Upstream and Downstream of bioprocess for the production of a amylase
  9. Estimation of volumetric liquid mass transfer coefficient (KLa) using sodium sulphite method.
  10. Preparation of immobilized enzymes & cells and evaluation of kinetic parameters.
  11. Computational Design of Fermentative Process for L-lysine production.

Text Books

  1. Practical Manual on Fermentation Technology by S. Kulandaivelu, S. Janarthanan 2012
  2. J.Jayaraman, “Laboratory Manual in Biochemistry”, New Age International Publications 2007
  3. Ejisenthal, R. &Danson N.J. (Eds) Enzyme Assays: “A Practical Approach”, IRI Press, Oxford, UK 1992.

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