FREE
AKTU Techniques in Biotechnology (BBT301) Syllabus PDF – B.Tech 2nd Year
Techniques in Biotechnology BBT301
All syllabus, notes, question bank & PYQ for this subject
Free
Download now
- English
- Semester 3
- 0 downloads
- 0 views
Official AKTU syllabus of Techniques in Biotechnology (BBT301) for B.Tech 2nd Year – 4 credits, L-T-P 3-1-0. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Techniques in Biotechnology
Download PDFBBT301 · 4 credits · L-T-P 3-1-0
Course Outcomes
| CO | Course Outcome |
|---|---|
| CO 1 | Acquire knowledge on types of microscope and its applications in Biotechnology. |
| CO 2 | Apply the principle of chromatographic techniques for qualitative and quantitative analysis of biomolecules. |
| CO 3 | Employ various spectroscopic techniques for qualitative and quantitative analysis of Bio- Molecules/Bio-Analytes. |
| CO 4 | Employ various electrophoresis and centrifugation techniques for analysis of Bio- Molecules/Bio-Analytes. |
| CO 5 | Acquire knowledge on 3 D printing, flow cytometry and biosensors. |
Detailed Syllabus
| Unit | Topic | Proposed Lectures |
|---|---|---|
| I | Light microscopy, Bright & Dark Field microscopy, Fluorescence microscopy, Phase Contrast microscopy, Electron microscopy: TEM and SEM, Atomic force microscopy and con focal scanning laser microscopy. Differential interference contrast microscopy. | 08 |
| II | Principle and Operations of Chromatography, Thin layer chromatography, lon Exchange Chromatography, High Performance Liquid Chromatography (HPLC), Gas Liquid Chromatography (GLC), Gel Filtration Chromatography, Affinity Chromatography. | 08 |
| III | Electromagnetic radiation and spectrum,Atomic absorption and Atomic emission spectroscopy,Principle, working and applications of UV-VIS, NMR, ESR and IR spectrometer, Principle and applications of Mass Spectroscopy, Circular Dichorism (CD) principles, Principle and applications of Positron Emission Tomography(PET), Basics of X-Ray diffraction analysis and their application in biotechnology . | 08 |
| IV | Theory of Electrophoresis, Factors affecting the migration of substances Gel electrophoresis, PAGE, SDS-PAGE, Agarose Electrophoresis of Nucleic Acid, Isoelectric Focusing of Protein Pulse Gel Electrophoresis and Western Blotting. Theory of centrifugation and sedimentation. Types of centrifuges, Preparative and analytical centrifugation; Density gradient centrifugation. Application of centrifugation for preparative and analytical purpose. | 08 |
| V | Principles of 3-D printing, 3-D Bioprinting of tissues, organs and bacteria.ldeal material properties for bioprinting, Biosensors: Principles and definition, characteristics of Ideal biosensors, Biochemical components of biosensors, Bioaffinity systems, Immunosensors. Principle and working of Flow Cytometry and cell sorter. | 08 |
Text Books
- Wilson, K, Walker, J., Principles and Techniques of Practical Biochemistry. 5th Ed. - Cambridge University Press,. Cambridge
- Sabari Ghosal&Anupama Sharma Awasthi., Fundamentals of Bioanalytical Techniques and Instrumentation, PHI learning
- Second edition (2018)
- Bioanalytical Techniques by A. Shourie and S SChapadgaonkar. TERI Press. 2015
- Immunoassay and Other Bioanalytical Techniques.Jeanette M. van Emon. CRC press. 2006
- 3D Bioprinting in Regenerative Engineering: Principles and Applications, Ali Knademhosseini&Gulden Camci-Unal, CRC
- Press (2018)
Ratings & Reviews
No reviews yet — share your experience with this item.