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AKTU Enzyme Engineering Lab (BBT453) Syllabus PDF – B.Tech 2nd Year
Enzyme Engineering Lab BBT453
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- Semester 4
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Official AKTU syllabus of Enzyme Engineering Lab (BBT453) for B.Tech 2nd Year – 1 credits, L-T-P 0-0-2. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Enzyme Engineering Lab
Download PDFBBT453 · 1 credits · L-T-P 0-0-2
Course Outcomes
| CO | Course Outcome |
|---|---|
| CO 1 | Exhibitthe extraction of enzyme and its enzyme activity |
| CO 2 | Perform purification and characterization of enzyme |
| CO 3 | Identity enzyme by enzyme assays |
| CO 4 | Perform enzyme immobilization and strain improvement for increased production |
| CO 5 | Comprehend the concept of enzyme inhibition. |
List of Experiments
- LIST OF EXPERIMENTS
- Production of commercially important enzymes from microbial sources.
- Isolation of alpha amylase from plant source
- Determination of enzyme activity and specific activity.
- Partial purification of isolated enzymes.
- Method of checking the purity of the enzyme -SDS-PAGE
- Characterization of enzymes-effect of pH , temperature and inhibitors on enzyme activity etc.
- Identification of Enzyme by different assay
- Purification of enzymes by different methods
- Immobilization of enzymes —Different Techniques such as adsorption, entrapment, encapsulation
- and crosslinking.
- Strain improvement techniques- physical, chemical and genetic manipulation methods.
- Formulation of enzyme stability.
- Enzyme inhibition
Text Books
- “An Introduction to Practical Enzyme Engineering”, Tata McGraw-Hill.
- R. Eisenthal and M.J. Dansen, “Enzyme Assays —A Practical Approach”, IRL Press, Oxford University Press, Oxford,
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