AKTU Pharmaceutical Biotechnology (Practical) (BP408P) Syllabus PDF – B.Pharm 4th Semester

Course Objectives

1. Provide fundamental knowledge of biotechnology concepts, including protein therapeutics, monoclonal antibodies, enzyme immobilization, and their pharmaceutical applications.
2. Explain the principles and applications of genetic engineering and recombinant DNA technology in pharmaceutical sciences.
3. Develop understanding of microbial biotransformation, fermentation processes, and large-scale production of pharmaceutical products such as alcohol, antibiotics, vaccines, and blood products.
4. Describe the principles, methodologies, delivery systems, and ethical considerations associated with gene therapy and modern vaccine technologies.
5. Introduce the regulatory requirements and standardization procedures for vaccines, sera, and other biopharmaceutical products.

Course Outcomes

Course Content

List of practical (Minimum 12 experiments must be performed)

1. Understanding Good microbiological laboratory practices while working in Microbiology laboratory aseptic handling in microbiology lab: growth and isolation of microbes using streaking, spreading and subculturing techniques: study of various equipments such as loop, straight wire, spreader, forceps, pipette, test tube, petridish, burner etc and apparatus used in microbiology lab- BOD incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer and microscopes.
2. Handling of biological spill, decontamination procedures and hygiene while handling microorganisms and disposal
3. Sterilization and evaluation of glassware and apparatus using hot air oven
4. Preparation and sterilization of solid and liquid culture medium using different techniques
5. Determination of microbiological efficacy of disinfectant/ preservative efficacy test
6. Tests for sterility of ophthalmic or parenteral marketed formulation according to IP.
7. Analysis of Biotechnological product (Protein, nucleic acid materials) by UV Vis and FTIR spectrophotometer
8. Production of alcohol using Fermentation process
9. Practice Whole cell immobilization technique (any one)
10. Practice Enzyme immobilization technique and its kinetics
11. Isolation and estimation of DNA and RNA
12. Isolation of plasmids and expression of protein
13. Agarose gel electrophoresis of DNA/ RNA
14. SDS polyacrylamide gel electrophoresis for proteins.
15. Microbiological assay of antibiotics.

Recommended References

1. Baltz, R. H., Davies, J. E. and Demain, A. L. Manual of Industrial Microbiology and Biotechnology. ASM Press.
2. Carter, S. J. CBS Publishers & Distributors.
3. Chou, S. L. M. Practical Biotechnology: A Guide to Biochemical Engineering. Springer.
4. Denyer, S. P., Hodges, N. A. and Gorman, S. P. Microbiology. Wiley-Blackwell.
5. Glick, B. R., Pasternak, J. J. and Patten, C. L. Molecular Biotechnology: Principles and Applications of Recombinant DNA. ASM Press.
6. Lachman, L., Lieberman, H. A., Kanig, J. L. and Khar, R. K. The Theory and Practice of Industrial Pharmacy. CBS Publishers & Distributors.
7. Pelczar, M. J., Chan, E. C. S. and Krieg, N. R. Microbiology. McGraw-Hill.