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AKTU Applied Chemistry for Biomolecules and Bioprocess Systems Lab (AAS152F / AAS252F) Syllabus PDF – B.Tech 1st Year
Applied Chemistry for Biomolecules and Bioprocess Systems Lab AAS152F / AAS252F
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Official AKTU syllabus of Applied Chemistry for Biomolecules and Bioprocess Systems Lab (AAS152F / AAS252F) for B.Tech 1st Year – 1 credits, L-T-P 0-0-2. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Applied Chemistry for Biomolecules and Bioprocess Systems Lab
Download PDFAAS152F / AAS252F · 1 credits · L-T-P 0-0-2
Course Objectives
- To identify and quantify biomolecules — carbohydrates, proteins, lipids, and nucleic acids
- To study enzyme kinetics and determine Km and Vmax for an enzyme-substrate system
- To analyse fermentation broth and study metabolic products using chemical methods
- To characterise biosensor responses and bioprocess media quality parameters
- To apply green chemistry principles in biosynthesis and bioprocess systems
List of Experiments
| Exp. | Theory | Title | Description / Objective Unit |
| 1 | Unit I | Qualitative Tests for Carbohydrates | Perform Molisch's, Benedict's, Fehling's, Barfoed's, and Seliwanoff's tests on given sugar solutions; identify reducing sugars, non-reducing sugars, monosaccharides, and polysaccharides |
| 2 | Unit I | Quantitative Estimation of Glucose by DNS Method | Estimate glucose concentration in unknown sample using 3,5-dinitrosalicylic acid (DNS) reagent; plot calibration curve; determine unknown concentration by UV-Vis spectrophotometry |
| 3 | Unit I | Estimation of Protein by Bradford / Lowry Method | Estimate protein concentration in a given sample using Bradford or Lowry method; plot BSA standard curve; determine protein content by colorimetry; relate to bioprocess broth analysis |
| 4 | Unit II | Effect of Temperature on Enzyme Activity | Study the effect of temperature (20°C– 80°C) on amylase or urease activity using starch-iodine or Nessler's method; determine optimum temperature; relate to bioreactor operating conditions |
| 5 | Unit II | Effect of pH on Enzyme Activity | Study effect of pH on amylase or protease activity using buffer solutions; determine optimum pH; relate to enzyme immobilisation and bioprocess pH control |
| 6 | Unit III | Estimation of Ethanol in Fermentation Broth | Determine ethanol content in fermentation broth by dichromate oxidation method or ebulliometer; relate to bioethanol fermentation process monitoring and yield calculation |
| 7 | Unit III | Estimation of Total Reducing Sugars in Fermentation Medium | Determine total reducing sugar content in fermentation medium before and after fermentation using DNS method; calculate substrate utilisation and fermentation efficiency |
| 8 | Unit IV | Biosensor Response – Glucose Biosensor Calibration | Calibrate a commercial glucose biosensor (glucometer) against standard glucose solutions; plot calibration curve; determine sensitivity and linearity; relate to enzyme biosensor working principle |
| 9 | Unit IV | Water Activity and pH of Fermentation Media | Measure pH and estimate water activity of different fermentation media; relate to microbial growth kinetics and substrate availability in bioprocess systems |
| 10 | Unit V | Green Synthesis of Silver Nanoparticles Using Plant Extract | Synthesise silver nanoparticles using neem or tulsi leaf extract as reducing and capping agent; confirm by UV-Vis spectroscopy (plasmon peak ~420 nm); relate to biogenic synthesis and green nanotechnology |
| 11 | Unit V | Estimation of Lipids by Soxhlet Extraction | Determine lipid content of a given biological sample (oilseed, yeast) by Soxhlet extraction using petroleum ether; calculate % lipid; relate to biodiesel feedstock evaluation |
| 12 | Unit I | Qualitative Tests for Amino Acids and Proteins | Perform Biuret, Ninhydrin, Xanthoproteic, and Millon's tests on amino acid and protein solutions; identify protein types; relate to downstream protein quality analysis |
Equipment / Chemicals Required
- UV-Vis spectrophotometer
- Analytical balance and centrifuge
- Water bath / thermostatic bath
- pH meter and buffer solutions
- DNS reagent, Bradford/Lowry reagent kits
- Soxhlet extraction apparatus
- Fermentation broth samples (prepared in advance)
- Commercial glucose biosensor (glucometer)
- Reflux condenser and COD glassware
- Microwave / water bath for nanoparticle synthesis
Teaching-Learning Methodology
Each laboratory session begins with a 10-minute pre-lab discussion connecting the experiment to the corresponding theory unit. Students record aim, principle, apparatus, chemicals, procedure, observation table, calculations, result, and inference in the laboratory record book. Post-lab discussion relates experimental findings to real-world engineering applications in the respective stream. Students maintain a laboratory record book assessed for viva, record, and day-to-day performance.
Course Outcomes
| COs | Course Outcome Statement | KL |
|---|---|---|
| CO1 | Perform qualitative and quantitative analysis of carbohydrates, proteins, and lipids | K3 |
| CO2 | Study effect of temperature and pH on enzyme activity and determine optimum conditions | K3 |
| CO3 | Estimate ethanol and reducing sugars in fermentation broth and calculate fermentation efficiency | K3 |
| CO4 | Calibrate a glucose biosensor and measure fermentation media quality parameters | K3 |
| CO5 | Synthesise silver nanoparticles by green route and determine lipid content by Soxhlet extraction | K3 |
Evaluation Pattern
| Sr. | Component | Marks | Weightage |
| 1. | Day-to-day performance (Observation, Procedure, Conduct) | 20 | 20% |
| 2. | Laboratory Record / File | 30 | 30% |
| 3. | End Semester Practical Examination (Experiment + Viva Voce) | 50 | 50% |
Text Books
- Engineering Chemistry – Jain and Jain
- Biochemistry – Lehninger, Nelson, and Cox
- Bioprocess Engineering Principles – Pauline M. Doran Safety and General Instructions
- Students must wear lab coat and safety goggles at all times in the chemistry laboratory
- Handle all acids, alkalis, and oxidising agents with extreme care; use fume hood for volatile chemicals
- Dispose of chemical waste in designated containers as per lab safety guidelines
- Do not pipette by mouth; use rubber bulb or mechanical pipette fillers
- Wash hands thoroughly after every experiment; report any spillage or accident immediately
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