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AKTU Basic Molecular Genetics Lab (BBI451) Syllabus PDF – B.Tech 2nd Year
Basic Molecular Genetics Lab BBI451
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- Semester 4
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Official AKTU syllabus of Basic Molecular Genetics Lab (BBI451) for B.Tech 2nd Year – 1 credits, L-T-P 0-0-2. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Basic Molecular Genetics Lab
Download PDFBBI451 · 1 credits · L-T-P 0-0-2
Course Outcomes
At the end of course, the student will be able to
| CO 1 | Remember principles of DNA structure, sources (plasmid, plant, bacterial) and isolation basics. | K3 |
| CO 2 | Explain the principles of electrophoresis, PCR, and spectrophotometry in DNA analysis. | K3 |
| CO 3 | Perform DNA isolation, amplification, and quantification using standard protocols. | K4 |
| CO 4 | Interpret agarose gel patterns and PCR products, | K4 |
| CO 5 | Applying knowledge of absorbance to quantify DNA concentration. | K3 |
Course Content
List of Experiments
- Preparation of buffers
- Preparation of different concentration of Agarose gel
- Horizontal Agarose gel electrophoresis
- Isolation of Plant genome
- Isolation of Bacterial genome
- Isolation of Plasmid DNA
- PCR amplification of DNA and visualization by gel electrophoresis
- Estimation of DNA by spectrophotometric method
- Estimation of Tm of duplex DNA strand using UV-Spectrophotometer
- Demonstration of DNA Sequencing Note: The instructor may add/delete/modify/tune experiments; (* two can be demonstration)
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