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AKTU Genomics & Proteomics (BBT071) Syllabus PDF – B.Tech 4th Year
Genomics & Proteomics BBT071
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Official AKTU syllabus of Genomics & Proteomics (BBT071) for B.Tech 4th Year – 3 credits, L-T-P 3-0-0. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Genomics & Proteomics
Download PDFBBT071 · 3 credits · L-T-P 3-0-0
Course Objectives
To teach basics of genomics and proteomics, their origin and applications. To develop understanding of fingerprinting and genome sequencing. To impart knowledge of peptide analysis and differential display proteomics. To demonstrate various techniques for high throughput analysis and protein sequencing. To brief about the emerging streams in genomics and proteomics, their techniques and applications. COURSE OUTCOME: After completion of this course successfully, the students will be able to:
| CO1 | Acquire the knowledge for genes, their origin and genome annotation techniques. | Understand |
| CO2 | To understand the basic concept of molecular markers, DNA fingerprinting and Genome projects. | Understand & Apply |
| CO3 | Apply the core proteomics principles for peptide fingerprinting and protein-protein interactions. | Create |
| CO4 | To impart knowledge of microarray, its variants, process, data analysis and pharmacogenomics. | Apply |
| CO5 | To understand the basic concepts of various new fields like metagenomics, metabonomics and systems biology. | Understand |
Course Content
| Unit | CONTANT | LECTURE |
|---|---|---|
| I. | Origin of genomes, Acquisition of new genes, DNA sequencing – chemical and enzymatic methods, The origins of introns, Genetics to genomics to functional genomics. Forward genetics (Phenotype to gene structure) and Reverse genetics (Gene structure to phenotype). Chromosome structure and Genome organization, Genome sequencing methods, Genome assembly, Gene identification methods, Sequences Comparison Techniques, Genome annotation techniques. | 10 |
| II. | Introduction to molecular markers-RFLP, RAPD, AFLP, SSRs and others. Genetic and physical maps, map based cloning, mapping population, southern and in situ hybridization for genome analysis, DNA fingerprinting; Single nucleotide polymorphisms, RNA interference, antisense RNA, siRNA, MiRNA, ; Human Genome Project; Pharmacogenomics: Ethical considerations of genetic testing; Genomics in drug discovery. | 10 |
| III. | Proteomics Protein analysis (includes measurement of concentration, amino- acid composition, N-terminal sequencing); 2-D electrophoresis of proteins; Micro-scale solution iso-electric focusing; Peptide fingerprinting; LC/MS-MS for identification of proteins and modified proteins; MALDITOF; SAGE and Differential display proteomics, Yeast two hybrid system. | 10 |
| IV. | Microarray, Pharmacogenetics High throughput screening in genome for drug discovery - identification of gene targets, Pharmacogenetics and drug development. | 8 |
| V. | Protein-protein and protein-DNA interactions; protein chips and functional proteomics; clinical and biomedical applications of proteomics; introduction to metabolomics, lipidomics, metagenomics and systems biology. | 8 |
| Total lectures | 46 |
Reference Books
| S.No | NAME OF AUTHORS/BOOKS/PUBLISHERS | YEAR OF PUBLICATION |
| 1. | Voet D, Voet JG & Pratt CW, Fundamentals of Biochemistry, 2nd ed. Wiley | 2006 |
| 2. | Introduction to Genomics Arthur M Lesk Oxford University Press | 2007 |
| 3. | Brown TA, Genomes, 3rd ed. Garland Science | 2006 |
| 4. | Campbell AM & Heyer LJ, Discovering Genomics, Proteomics and Bioinformatics, 2nd ed. Benjamin Cummings | 2007 |
| 5. | Primrose S & Twyman R, Principles of Gene Manipulation and Genomics, 7th ed, Blackwell | 2006 |
| 6. | Glick BR & Pasternak JJ, Molecular Biotechnology, 3rd ed, ASM Press | 1998 |
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