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AKTU Bioseperation & Down Stream Processing (BBT072) Syllabus PDF – B.Tech 4th Year
Bioseperation & Down Stream Processing BBT072
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Official AKTU syllabus of Bioseperation & Down Stream Processing (BBT072) for B.Tech 4th Year – 3 credits, L-T-P 3-0-0. Read the unit-wise course content, course outcomes, evaluation pattern and recommended books online, or download the PDF.
Syllabus — Bioseperation & Down Stream Processing
Download PDFBBT072 · 3 credits · L-T-P 3-0-0
Course Objectives
The major objective of this course is to impart in students the skills to operate bioprocesses for production of various Bio-products This course is formulated to teach various methods of product separation, isolation and purification To teach the construction of genomic c-DNA libraries, cloning and strain improvement COURSE OUTCOME: After successful completion of the course the students will be able to:
| CO1 | To operate, design and optimize the production medium, they will gain the ability to handle bioreactors to carry out different separation processes involving various types of bioproducts. | Understand & Analyze |
| CO2 | The students will be skilled in choosing a process of separation for a particular product, they will know how to design the relevant equipment, calculate the yield, and degree of purification. | Understand, Analyze & Create |
Course Content
| Units | CONTENT | LECTURE |
|---|---|---|
| I. | INTRODUCTION TO BIOSEPARATION PROCESS: Role and importance of bioseparation in biotechnological processes: RIPP scheme, Problems and requirements of bioproducts purification - Properties of Biomolecules - Characteristics of fermentation broth - Biological activity, Analysis of purity-Process economics: Capital and operating cost analysis. | 8 |
| II. | REMOVAL OF INSOUBLES: Cell disruption methods for intracellular products: Physical, chemical and mechanical - Removal of insolubles: Biomass and particulate debris separation techniques - flocculation - sedimentation - centrifugation and filtration methods. | 8 |
| III. | ISOLATION OF PRODUCTS: Adsorption: Principles - Langumir - Freundlich isotherms - Extraction: Basics- Batch and continuous, aqueous two-phase extraction - supercritical extraction - in situ product removal - Precipitation: Methods of precipitation with salts - organic solvents and polymers - Membrane based separations: Micro and ultra filtration - theory - design and configuration of membrane separation equipments and its applications. | 8 |
| IV. | PURIFICATION OF BIOPRODUCTS: Basic principles of Chromatographic separations: GC-HPLC - gel permeation - ion- exchange - affinity - reverse phase and hydrophobic interaction chromatography - Electrophoretic separation techniques: capillary - isoelectric focusing-2D gel electrophoresis - Hybrid separation technologies: GC-MS and LC-MS. | 8 |
| V. | PRODUCT POLISHING: Crystallization: Principles-Nucleation- Crystal growth-Kinetics-Batch crystallizers: Scale-up and design, Drying: PrinciplesWater in biological solids- Heat and mass transfer- Drying equipments: description and operation-Vacuum shelf - rotary dryer-Freeze dryer-Spray dryer. Biomolecules of Commercial importance Ethanol, citric acid, lysine, steroids, penicillin, dextran, trehalose, subtilisin, chymosin, vitamin B12, hepatitis B vaccine, insulin, erythropoietin, monoclonal antibodies. | 8 |
| Total lectures | 40 |
Reference Books
| S.No | NAME OF AUTHORS/BOOKS/PUBLISHERS | YEAR OF PUBLICATION |
| 1. | Raja Ghosh, “Principles of Bioseparations Engineering”, World Scientific Publishing | 2006 |
| 2. | Ladisch. M. R, "Bioseparation Engineering: Principles, Practice and Economics", John Wiley & sons, New York | 2001 |
| 3. | Asenjo.J.M, "Separation processes in Biotechnology" Marcel Dekker Inc | 1993 |
| 4. | Bailey & oils, Biochemical Engg. Fundamentals, McGraw-Hill | 1990 |
| 5. | Roger G. Harrison, Paul Todd, Scott R. Rudge, Demetri P. Petrides, "Bioseparation science and Engineering" Oxford University press. | 2003 |
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